CellShot Partitioned Flow Electroporation simplifies electroporation, allowing cells to be transfected directly in their culture media without washing or mixing. The CellShot System handles the entire process, resulting in efficient and effective nonviral cell and gene therapy. The System removes the need for EP buffers, minimizing cell damage, loss, and manual processes. The technology results in high transfection efficiency and viability and is suitable for R&D and clinical purposes.