Platform

A platform for safe, scalable, and
controlled non-viral gene delivery

CellShot integrates proprietary electroporation technology, system engineering, and process-ready design to enable damage-minimized, scalable gene transfer—from research to GMP manufacturing.

CellShot^® Suite

An Integrated-Modular-System, cGMP-Ready End-to-End Platform for Non-Viral Cell Engineering

CellShot® Suite integrates hardware, consumables, protective buffers, and digital workflows into a single non-viral cell engineering platform — enabling safe, reproducible, and cGMP-ready manufacturing from CRISPR to cell therapy.

CellShot Suite Integrated System Diagram

CellShot^® Suite is an integrated non-viral cell engineering platform that combines automation, safety, and data intelligence into a single modular system.
Built around five core modules — Controller, Cartridge, Proliva™, Cryoliva™, and Database — it enables consistent, scalable, and regulator-ready cell engineering workflows.

Registered under a US FDA Drug Master File (DMF #30803), CellShot® Suite supports clinical and manufacturing use across CRISPR, mRNA, and RNAi applications.

Every process is automated, monitored, and digitally recorded, enabling advanced QC, full traceability, and error-minimized cGMP manufacturing for next-generation cell and gene therapies.

The CellShot® ND-FFT design philosophy is realized through five core modules.

ND-FFT Core Element	CellShot Module	How It Is Implemented (Design Level)	Resulting Value
Electrical Control Focused & Uniform Field	CellShot Controller	Precision high-voltage pulse shaping Field-focused electrode geometry control Protocol-driven voltage & timing execution	Efficient non-viral delivery Reduced membrane over-stress Consistent transfection across cell types
Thermal Control Active thermodynamic defense	CellShot Controller CellShot Cartridge	60W Peltier cartridge cooling Real-time IR temperature monitoring Heat-dissipating cartridge architecture	Prevention of Joule heating damage Preserved viability & functionality Stable post-EP recovery
Buffer Control Electrochemical & biochemical optimization architecture	CellShot Proliva™ CellShot Cryoliva™	Membrane-protective EP buffer design Osmotic & ionic stress mitigation Low-DMSO cryo-compatible chemistry	High post-EP viability Sustained proliferation & expansion Repeatable multigenic delivery
Flow Control Scalable & high-throughput	CellShot Cartridge Pumping System	Pressure-sensing syringe pumping Microfluidic flow uniformity Batch-scalable cartridge formats	Reproducible processing High-throughput manufacturing Reduced batch-to-batch variability
System Integration Digital orchestration	CellShot Database	Protocol-driven execution Cartridge ID & traceability Automated data capture & audit trail	Full process reproducibility cGMP-ready documentation Error-minimized manufacturing

01CellShot Controller

The CellShot Controller - currently represented by the FDA DMF-registered CellShot Badger - is the core engine of the platform. Following the work instructions stored in the CellShot Database, the Controller initializes the loaded cartridge, regulates cooling, applies high-voltage pulses, and performs precision pumping. This integration ensures damage-free, reproducible nonviral gene transfer with consistent quality across diverse cell types.

TMS

(Temperature Management System) ensures precise thermal control throughout electroporation. Cartridge cooling is managed by a feedback-controlled block, while an integrated IR sensor directly monitors the cell compartment. Thisdual-feedback design secures optimal thermal conditions even with mounting errors or malfunctions.

HVS

(High-Voltage Supply) is fully enclosed for safety. High-voltage connections activate only after the cartridge is securely mounted, physically isolating the user from risk. HEPA-filtered internal circulation maintains operational stability.

The Turtle Dispenser

is an optional accessory for the CellShot Badger Device, enabling high-throughput screening of electroporation conditions. Two versions are available: Turtle-I for 96-well plates and Turtle-II for standard E-tubes.

Conditions can be selected from validated screening libraries or custom-programmed via the CellShot Database, supporting flexible research needs. Ideal for R&D, streamlining electroporation optimization.

02CellShot Cartridge

The CellShot Cartridge provides the single-use consumable format that ensures sterility and GMP compliance. Two formats are available: standard syringe-based (3 mL, 5 mL, 10 mL) and high-capacity cell-bag type (2026 release), allowing scaling from research to commercial production.

CAT NO.	FCC-2310	FCC-2312	FCC-3351	FCC-3391
Cell Volume	2ml	3ml	5ml	10ml
Max Capa.	2x10⁸ cells	3x10⁸ cells	5x10⁸ cells	1x10⁹ cells
Mat Volume	2ml	3ml	5ml	5ml
Proc. Time	< 2min	< 3min	< 4min	< 8min
DMF	MF 30803	MF 30803	MF 30803	MF 30803

03CellShot Proliva™

Proliva™ Buffer Architecture

"Proliva™ is the biochemical protection layer of the CellShot ND-FFT system—designed to preserve cell health not only during electroporation, but throughout recovery and expansion."

Biochemical protection engineered for non-destructive cell processing

In non-viral gene delivery, cellular stress does not end at membrane permeabilization.
Electrochemical reactions, oxidative stress, and mitochondrial dysfunction continue to affect cell health.

Proliva™ is not a single buffer—it is a modular biochemical protection architecture, engineered to preserve cell viability and functionality.

Design Philosophy – Buffer as Architecture

Beyond a Single Buffer: Proliva™ is designed as a system, not a formulation.

Conventional electroporation buffers focus on conductivity and osmolarity. Proliva™ takes a different approach to:

Protect cellular membranes and organelles
Suppress electrochemical and oxidative stress
Support immediate post-EP recovery
Maintain compatibility with GMP manufacturing

Each Proliva™ component is positioned at a specific stage of the ND-FFT process, forming a continuous defense layer.

Specs	PROLIVA™ CH21 FCB-CH21	PROLIVA™ MH21 FCB-MH21	PROLIVA™ ENG1 FCB-ENG1	PROLIVA™ MNAC FCB-MNAC	PROLIVA™ RPMN FCB-RPMN	PROLIVA™ RDEX FCB-RPMN
Role	Core EP C-Buffer (Cell & Gene Resuspension)	Electrochemical Protection M-Buffer	Optional C-Buffer Additive (Stress Recovery)	Optional M-Buffer Additive (Electrochemical Defense)	Recovery Medium Additive (Post-EP Biochemical Support)	Recovery Medium Additive (24h 2nd Recovery Support)
Primary Function	Protects cell membranes and organelles during electroporation Mitigates ROS and supports mitochondrial recovery	Stabilizes electrochemical environment Reduces field-induced reactions and thermodynamic stress	Enhances mitochondrial protection (ΔΨm) Supports ROS recovery post-EP	Neutralizes metal ions Provides additional electrochemical protection	Supports metabolic and functional recovery Optimized for 30 min instant recovery and 24 h post-EP culture	Supports NF-κB, AP-1 suppression Optimized for 24 h post-EP culture
System Position	Essential execution buffer	Essential protection buffer	Recovery-enhancing supplement	Protection-enhancing supplement	Essential recovery supplement	Protection recovery supplement
Usage	Standard: CH21 Strong EP: CH22 Max EP: CH23	Standard: MH21 Strong EP: MH22 Max EP: MH23	Add 1% (v/v) to C-buffer	Add 0.4–1% (v/v) to M-buffer	Add 1% (v/v) to recovery medium	Add 0.025% (v/v) to culture medium
Compatibility	All cell types	All cell types	All cell types	All cell types	Recovery media (e.g., CTL medium)	All types of culture media
Storage	2–8 °C	2–8 °C	–80 °C (deep freeze)	–80 °C (deep freeze)	–80 °C (deep freeze)	–80 °C (deep freeze)
Product

Biochemical Protection Aligned with Physical Control

Proliva™ is tightly integrated with the four core control dimensions of ND-FFT:

Electrical Control
Maintains membrane stability under focused electric fields
Thermal Control
Supports mitochondrial integrity under controlled electroporation
Flow Control
Enables consistent biochemical exposure under continuous flow
Buffer Control
Provides electrochemical defense at each process stage

This integration enables:

● High post-electroporation viability
● Preserved proliferation capacity
● Repeatable transfection workflows
● Manufacturing-scale robustness

04CellShot Cryoliva™

Cryoliva™: Preservation Is a Process, Not a Bottle

Cryoliva™ is currently under development as a process-optimized cryopreservation interface, designed specifically for cells conditioned by CellShot Proliva™ and ND-FFT electroporation.

Cryoliva™ is engineered to preserve the cellular state established during non-destructive gene delivery—minimizing toxicity, maintaining mitochondrial integrity, and enabling low-DMSO workflows.

Low-DMSO cryopreservation is not a formulation feature — it is a cell state. Cryoliva™ is designed to preserve that state.

Why Cryoliva™ Is Different

Conventional cryopreservation media often rely on high DMSO to compensate for damage. In contrast, CellShot Proliva™ enables diverse protection reagents loading during electroporation, stabilizing cells before freezing begins.

✓
Preserve Proliva-conditioned intracellular protection reagents
✓
Reduce reliance on cytotoxic cryoprotectants

✓
Maintain post-thaw proliferation and recovery
✓
Support seamless transition from recovery to freezing

As an option, commercial low-DMSO cryomedia can be directly applied following Proliva-based electroporation, already demonstrating high post-thaw recovery and workflow compatibility.

05CellShot Database(www.cellshotdb.com)

Digital Orchestration Layer for ND-FFT Manufacturing

CellShot Database Cloud Integration Diagram

CellShot Database: The Digital Backbone of the CellShot Platform

CellShot Database is the cloud-based digital backbone of the CellShot® platform, designed to orchestrate, monitor, and validate every step of the ND-FFT non-viral gene delivery workflow.

Built on a secure AWS infrastructure, CellShot Database integrates electrical control, cartridge configuration, process execution, device monitoring, and data analytics into a single unified system—transforming electroporation from a manual operation into a fully traceable, protocol-driven manufacturing process.

End-to-End Workflow Integration

CellShot Database connects directly with each CellShot Controller to enable:

Energy Spectrum design & protocol definition
Cartridge configuration and compatibility management
Automated task execution
Real-time device monitoring
Result analysis and reporting

All process parameters—including voltage, current, temperature, pressure, flow rate, and processing time—are continuously captured, analyzed, and archived.

Core Functional Modules

•

Energy Spectrum Designer: Design, store, and reuse validated electroporation energy profiles

•

Cartridge Designer: Configure cartridge formats, flow paths, and process compatibility

•

Task Manager: Execute protocol-driven operations with minimized operator variability

•

Device Monitoring: Real-time tracking of system status, alarms, and performance metrics

•

Dashboard & Reports: Batch-level summaries, trend analysis, and performance benchmarking

Manufacturing-Grade Data Integrity

Full process traceability
Audit-ready data logging
Batch-to-batch reproducibility
Multi-site network scalability

Advanced analytics modules further enable reagent/library management, automated FACS data integration, and custom dashboards for both R&D optimization and cGMP manufacturing control.

Why the Database Matters

ND-FFT is not a single device or buffer—it is a system-level manufacturing philosophy.

CellShot Database completes this system by ensuring that every physical process is digitally defined, executed, and verified.

Non-viral gene delivery becomes manufacturing-ready only when physics, chemistry, hardware, and data are unified.